17+ Ligation Calculator
These links can help you calculate how much insert and backbone you need for different types of cloning methods such as restriction. Purpose Key Facts Contents Vector size Vector amount.
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Are you carrying out the ligation reaction under high salt conditions 100 mM NaCl.
. Please enter the molar vector. DNA Name ds DNA size in base pairs bp Quantity in micogram ug PART 2. Enter 2 kb for your DNA insert length and 6 kb for your vector DNA length making sure that the units selected are correct.
Convert double strand DNA ds DNA to pmoles and ends. Options include conversion of mass to moles ligation amounts conversion of OD. For example in NEBuffer 31.
Web Calculators NEBioCalculator Use this tool for your scientific calculations and conversions for DNA and RNA. Refer to the text below the tool for more. Single base 1 nt TA overhangs.
Web Calculating the optimal amounts of vector and insert to use in the In-Fusion Cloning reaction is easy with our In-Fusion molar ratio calculatorsimply provide the size of your insert. Web The NEBioCalculator Ligation module can help you plan a successful ligation reaction as part of a restriction cloning workflow by optimizing the molar ratio between your vector. Web Download the Ligation Calculator app for Android Android 23 Gingerbread Android 22 Froyo Total Reaction Mixµl Volume of the bufferµl Volume of the enzymeµl Vector.
100ng vector 05kb insert. Options include conversion of mass to moles ligation amounts. Web First click on the ligation calculator module.
Web Ligation Calculator Determines the optimal insert amount for a cloning reaction based on vector and insert properties. Web Cloning ligation reaction calculators. Web Ligation Calculator Determines the optimal insert amount for a cloning reaction based on vector and insert properties.
Insert size in bp. Web LIGATION CALCULATOR Please provide the following information. Vector amount in ng.
Use this tool for your scientific calculations and conversions for DNA and RNA. Web How much 500bp insert DNA needs to be added to 100ng of 30kb vector in a ligation reaction for a desired vectorinsert ratio of 13. Vector size in bp.
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